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Monday 22 October 2007

Spectrofluorimetric Study and Detection of Ketoconazole in the Presence of beta-cyclodextrin.

By: Jalali F, Afshoon A.

J Fluoresc 2007 Oct;(): [Epub ahead of print]

The formation of a complex between ketoconazole and beta-cyclodextrin was followed by spectrofluorimety. The inclusion of ketoconazole in beta-cyclodextrin cavity enhanced the native fluorescence of the drug. The stoichiometry of the complex was 1:1 beta-cyclodextrin to ketoconazole and the stability constant of the complex (log K (f)) was determined to be 4.3 +/- 0.01 at pH = 7.9 and 3.7 +/- 0.04 at pH = 2.6. A sensitive spectrofluorimetric method for the detection of ketoconazole is presented. At optimized experimental conditions, a linear relationship between the fluorescence intensity of the solution and concentration of ketoconazole is observed in the range of 0.01-10 mug ml(-1) (5 x 10(-8) M-1.88 x 10(-5) M). The method was applied to the detection of ketoconazole in pharmaceutical products and the results were satisfactory in comparison to the official method (relative error = 2.8% and standard deviation = 0.06 for tablets of ketoconazole). The recovery of ketoconazole from a blood serum sample, determined by the proposed method, was 97.1 +/- 2.4%.

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